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What is the heritable component of spinal deformities in the European sea bass (Dicentrarchus labrax)? ArchiMer
Bardon, Agnes; Vandeputte, Marc; Dupont-nivet, Mathilde; Chavanne, Herve; Haffray, Pierrick; Vergnet, Alain; Chatain, Beatrice.
Malformations in farmed fish are a prime research topic as it has a major impact on fish welfare and on the economical value of the product. In this study, the genetic component of spine deformities was estimated in European sea bass, a major species of Mediterranean aquaculture, with the use of a posteriori reconstruction of pedigree with microsatellites. Our population exhibited a very high malformation rate (81%),probably due to intense swimming of fish during pre-growing The heritability of global spine deformities was evaluated to be 0.21 +/- 0.04 on the underlying scale (033 +/- 0.06 for lordosis, 0.13 +/- 0.04 for scoliosis). Deformities scored by an internal or external examination turned out to be genetically the same trait (genetic...
Tipo: Text Palavras-chave: Genetic correlations; Heritability; Growth; Deformities; Dicentrarchus labrax; European sea bass.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6768.pdf
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Genetic Parameters and Genome-Wide Association Studies of Quality Traits Characterised Using Imaging Technologies in Rainbow Trout, Oncorhynchus mykiss ArchiMer
Blay, Carole; Haffray, Pierrick; Bugeon, Jérôme; D’ambrosio, Jonathan; Dechamp, Nicolas; Collewet, Guylaine; Enez, Florian; Petit, Vincent; Cousin, Xavier; Corraze, Geneviève; Phocas, Florence; Dupont-nivet, Mathilde.
One of the top priorities of the aquaculture industry is the genetic improvement of economically important traits in fish, such as those related to processing and quality. However, the accuracy of genetic evaluations has been hindered by a lack of data on such traits from a sufficiently large population of animals. The objectives of this study were thus threefold: (i) to estimate genetic parameters of growth-, yield-, and quality-related traits in rainbow trout (Oncorhynchus mykiss) using three different phenotyping technologies [invasive and non-invasive: microwave-based, digital image analysis, and magnetic resonance imaging (MRI)], (ii) to detect quantitative trait loci (QTLs) associated with these traits, and (iii) to identify candidate genes present...
Tipo: Text Palavras-chave: Aquaculture; Fat content; Flesh colour; Magnetic resonance imaging; Fatmeter; Computer vision; Genetic correlations; QTL.
Ano: 2021 URL: https://archimer.ifremer.fr/doc/00682/79410/81958.pdf
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Genetic variability and selective breeding for traits of aquacultural interest in the Pacific oyster (Crassostrea gigas) ArchiMer
Boudry, Pierre; Degremont, Lionel; Taris, Nicolas; Mccombie, Helen; Haffray, Pierrick; Ernande, Bruno.
The most significant genetic improvement for production of Pacific oyster (Crassastrea gigas) has been obtained through the breeding of triploids, especially since the development of tetraploids. Quantitative genetics studies suggest that significant gains, for disease resistance or for other traits of aquacultural interest, could be obtained using this approach. However, the limited extent of hatchery-propagation (versus natural recruitment) and/or various technical difficulties and biological characteristics of the species have slowed the development of selective breeding programs. Recently, in the USA, Australia and New Zealand, family-based selective breeding programs have been initiated to improve growth and yield. In Europe, where both natural and...
Tipo: Text Palavras-chave: Microsatellites; Quantitative genetic; Genetic variability; Crassostrea gigas; Oysters.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-2861.pdf
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Genetic variability and selective breeding for traits of aquacultural interest in the Pacific oyster (Crassostrea gigas) (slides) ArchiMer
Boudry, Pierre; Degremont, Lionel; Taris, Nicolas; Mccombie, Helen; Haffray, Pierrick; Ernande, Bruno.
To date, the most significant genetic improvement for the production of Pacific oyster (Crassostrea gigas) has been obtained through the breeding of triploids, especially since the development of tetraploids. Quantitative genetics studies suggest that significant gains, for disease resistance or for other traits of aquacultural interest, could be obtained in diploids using this approach. However, the limited extent of hatchery-propagation (versus natural recruitment) and/or various technical difficulties and biological characteristics of the species have retarded the development of selective breeding programs. Recently, in the U.S.A., Australia and New Zealand, countries where hatcheries are a major source of C. gigas juveniles, family-based selective...
Tipo: Text Palavras-chave: Microsatellite markers; Selective breeding; Genetic; Genetic variability; Crassostrea gigas; Pacific oysters.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/acte-3449.pdf
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Research and development for the genetic improvement of the Pacific oyster in France : present and prospects ArchiMer
Boudry, Pierre; Haffray, Pierrick.
The most significant genetic improvement for the production of Pacific oyster (Crassostrea gigas) in France to date has been through the breeding of triploids, especially since the development of tetraploids. Triploid oysters commonly resist summer mortality better than diploids. This is most probably because reproductive allocation is so important in this phenomenon. Quantitative genetics studies strongly suggest that a significant gain in resistance to summer mortality, or other traits of interest, could be obtained by selective breeding. Practical difficulties however, and the high cost of breeding large numbers of families under common conditions over many generations remain major constraints to family-based selective breeding in oysters. In the last...
Tipo: Text Palavras-chave: Polyploidization; Selective breeding; Genetic; Crassostrea gigas; Pacific oysters.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/acte-3438.pdf
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Projet CRYOYSTER: Optimisation, standardisation et validation de la congélation de laitance d'huître creuse Crassostrea gigas à des fins de conservation et de diffusion génétique - Appel d'offre de l'OFIMER du 8 Novembre 2001 - Rapport final ArchiMer
Brizard, Raphael; Bernardi, Maud; Boudry, Pierre; Haffray, Pierrick; Labbe, Catherine; Maisse, Gérard; Maurouard, Elise; Robert, Rene; Roger, Jean-luc.
Afin d'appuyer la filière ostréicole, les programmes de recherche actuels visent à proposer des méthodes d'amélioration génétique des rendements et de la qualité du produit (sélection, polyploïdisation). A l'image des productions animales terrestres, la continuation de ces travaux et la diffusion du progrès génétique dans la filière seraient grandement facilitée par le développement de techniques de conservation des génotypes sélectionnés. La cryopréservation qui est utilisée fréquemment dans les programmes de sélection des animaux terrestres et des espèces piscicoles n'est toujours pas disponible pour les mollusques bivalves. Cette technique présente les atouts qui peuvent pourraient satisfaire les différents acteurs de la filière ostréicole car elle...
Tipo: Text Palavras-chave: Congélation sperme; Standardisation; Procédure; Ecloserie expérimentale; Fécondation; Cryopréservation; Sperme cryopréservé.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/00049/16056/13516.pdf
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Flesh quality in large rainbow trout with high or low fillet yield ArchiMer
Bugeon, Jerome; Lefevre, Florence; Cardinal, Mireille; Uyanik, Ayhan; Davenel, Armel; Haffray, Pierrick.
Rainbow trout with different fillet yield [56 and 65% for low (LY) and high yield (HY), respectively] were examined for muscle organization and flesh quality (instrumental and sensorial evaluations). Both groups had similar body weight (3.6 kg in mean), but the HY group had a higher carcass yield (+15%). Higher total muscle area in the HY group (+20%) was associated with a higher number of muscle fibers (+22%). Flesh of the HY group presented a higher area of myosepta (+10%), fat content (+10%) and luminosity (+6%). Raw fillet mechanical resistance was higher for the HY group; an opposite result was obtained for cooked flesh. Sensorial evaluation of cooked flesh revealed no important differences between groups. Smoked fillet from the HY group presented...
Tipo: Text
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00014/12532/9532.pdf
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Side effects of sexual maturation on heritability estimates in rainbow trout (Oncorhynchus mykiss) ArchiMer
Dupont-nivet, Mathilde; Chevassus, Bernard; Mauger, Stephane; Haffray, Pierrick; Vandeputte, Marc.
Tipo: Text Palavras-chave: Fish; Genetic parameters; Sex effect; Growth.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00016/12690/9656.pdf
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Genotype by environment interactions for growth in European seabass (Dicentrarchus labrax) are large when growth rate rather than weight is considered ArchiMer
Dupont-nivet, Mathilde; Karahan-nomm, Bilge; Vergnet, Alain; Merdy, Olivier; Haffray, Pierrick; Chavanne, Herve; Chatain, Beatrice; Vandeputte, Marc.
Two hundred fifty three full-sib families from 33 males and 23 females of European seabass were produced in a partly factorial mating design. All fish were reared in the same tank for 14 months until reaching mean weight of 35 g. then 7000 of them were individually tagged and weighed, and dispatched to four farms in different locations (France, Israel, Italy and Portugal) representing a wide variety of environmental conditions. Around mean weight of 400 g. 1177 to 1667 fish at each site were weighed. Daily growth coefficient (DGC) was calculated. Pedigrees were successfully redrawn for 99.2% of fish using microsatellite markers. Genetic correlations between sites were high for body weight (>0.80 in all cases but one, i.e., five cases over six), but only...
Tipo: Text Palavras-chave: Dicentrarchus labrax; Seabass; Growth; Daily growth coefficient; Heritability; G x E interactions; Aquaculture.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00011/12256/9227.pdf
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Improvement of chilled seabass sperm conservation using a cell culture medium ArchiMer
Fauvel, Christian; Boryshpolets, Sergey; Cosson, J.; Leedy, J. G. Wilson; Labbe, C.; Haffray, Pierrick; Suquet, Marc.
The sperm of seabass is very fragile and it quickly loses its ability to fertilize after collection either if kept undiluted or in classic saline media. In order to avoid cryopreservation when only short conservation is required, the process of sperm management including sperm collection, sperm dilution rate in storage medium and storage medium composition, was subject to experimental trials. A concentration of 20% urine generated a low pH of seminal fluid, and it immediately altered the motility ability. However, pH did not seem to be the key agent of motility prevention since sperm dilution in Leibovitz culture medium (L15) or in classic saline medium both presenting a similar low pH (7.3) did not affect motility. L15 increased the duration of sperm...
Tipo: Text
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00111/22221/20066.pdf
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How to genetically increase fillet yield in fish: New insights from simulations based on field data ArchiMer
Fraslin, Clemence; Dupont-nivet, Mathilde; Haffray, Pierrick; Bestin, Anastasia; Vandeputte, Marc.
Fillet yield (i.e. the proportion of edible flesh from a given amount of fish) is a trait of high economic interest for species sold as fillets. Improving it by selective breeding is not an easy task, as it is a ratio trait, which causes mathematical difficulties. It is moreover a specific ratio where numerator (fillet weight) and denominator (body weight) are very strongly correlated (genetic and phenotypic correlations in the range 0.89–0.99). This led some authors to conclude that they have the same genetic and phenotypic basis, precluding selection for improved fillet yield. In this study, we propose to study fillet yield as a component of two traits, fillet weight and waste weight (waste weight = body weight-fillet weight, so the sum of head, bones,...
Tipo: Text Palavras-chave: Aquaculture; Genetics; Heritability; Selection; Processing yields.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00415/52599/53430.pdf
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Metapopulation patterns of additive and nonadditive genetic variance in the sea bass ( Dicentrarchus labrax ) ArchiMer
Guinand, Bruno; Vandeputte, Marc; Dupont-nivet, Mathilde; Vergnet, Alain; Haffray, Pierrick; Chavanne, Herve; Chatain, Beatrice.
Describing and explaining the geographic within-species variation in phenotypes (“phenogeography”) in the sea over a species distribution range is central to our understanding of a variety of eco-evolutionary topics. However, phenogeographic studies that have a large potential to investigate adaptive variation are overcome by phylogeographic studies, still mainly focusing on neutral markers. How genotypic and phenotypic data could covary over large geographic scales remains poorly understood in marine species. We crossed 75 noninbred sires (five origins) and 26 dams (two origins; each side of a hybrid zone) in a factorial diallel cross in order to investigate geographic variation for early survival and sex ratio in the metapopulation of the European sea...
Tipo: Text Palavras-chave: Additive and nonadditive variance; Darwin's corollary; Heterosis; Mito-nuclear epistasis; Pleiotropy.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00376/48759/49154.pdf
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Negative genetic correlations between production traits and head or bony tissues in large all-female rainbow trout (Oncorhynchus mykiss) ArchiMer
Haffray, Pierrick; Bugeon, Jerome; Pincent, Cedric; Chapuis, Herve; Mazeiraud, Emmanuel; Rossignol, Marie-noelle; Chatain, Beatrice; Vandeputte, Marc; Dupont-nivet, Mathilde.
Genetic parameters of production traits (growth, carcass yield, fillet yield) and bony tissues (head and vertebral axis) were estimated for large all-female rainbow trout Oncorhynchus mykiss reared in freshwater. Genetic parameters were estimated using REML at 16 months of age (1636 g) on 1962 DNA-assigned progenies from a partial factorial mating design with 60 dams and 100 sex-reversed sires. Most traits presented medium to high heritability (0.37 to 0.54). A high genetic correlation (r(A)=0.97) was found between fillet yield and headless gutted carcass yield (or HGCarc%). Due to its higher heritability and high genetic correlation with fillet yield, selection on HGCarc% should result in a 50% increase in selection efficiency on fillet yield by reducing...
Tipo: Text Palavras-chave: Resource allocation; Fillet yield; Head; Salmonids; Aquaculture; Selective breeding.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00109/22042/19801.pdf
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Genetic parameters of in-vivo prediction of carcass, head and fillet yields by internal ultrasound and 2D external imagery in large rainbow trout (Oncorhynchus mykiss) ArchiMer
Haffray, Pierrick; Bugeon, Jerome; Rivard, Quentin; Quittet, Benjamin; Puyo, Sophie; Allamelou, Jean Michel; Vandeputte, Marc; Dupont-nivet, Mathilde.
Selection to improve processing yields relies on sib selection, in which live candidates are ranked according to their family breeding value. This approach limits genetic progress, as it only exploits genetic variability between families and not within them. Indirect criteria measured on live candidates could overcome this limitation. The present study (1) proposes a procedure to identify indirect criteria to predict processing yields in rainbow trout (head, carcass and fillet yields), (2) estimates genetic parameters of these indirect criteria, and (3) predicts relative genetic gains in processing yields using full-sib selection or indirect individual selection on those indirect criteria. DNA-pedigreed all-female rainbow trout Oncorhynchus mykiss (n =...
Tipo: Text Palavras-chave: Salmonids; Heritability; Fillet yield; Selection; Aquaculture; Morphometry.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00157/26847/25249.pdf
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Accuracy of BLUP breeding values in a factorial mating design with mixed families and marker-based parentage assignment in rainbow trout Oncorhynchus mykiss ArchiMer
Haffray, Pierrick; Enez, Florian; Bugeon, Jerome; Chapuis, Herve; Dupont-nivet, Mathilde; Chatain, Beatrice; Vandeputte, Marc.
Marker-based parentage assignment provides the opportunity to investigate factors of efficiency for mixed-family designs and factorial mating. In such designs, family size is both uncontrolled and small, which may be thought to limit the accuracy of estimated breeding values (EBVs). The objective of this work was to estimate the accuracy of EBVs of growth and quality traits in a large factorial mating design and in commercial breeding conditions. An expected six hundred full-sib families of rainbow trout Oncorhynchus mykiss (2042 fish in total) were produced by ten factorial matings of six dams with ten sires. Fish were phenotyped for body weight, carcass yield, fillet yield, fillet fat content and fillet colour, and family information was recovered using...
Tipo: Text Palavras-chave: Aquaculture; Salmonids; DNA parentage assignment; Sib selection; EBV accuracy.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00429/54062/55395.pdf
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European sea bass - Dicentrarchus labrax ArchiMer
Haffray, Pierrick; Tsigenopoulos, C.s.; Bonhomme, Francois; Chatain, Beatrice; Magoulas, Antonio; Rye, M; Triantafyllidis, A; Triantafyllidis, C.
Tipo: Text
Ano: 2007 URL: http://archimer.ifremer.fr/doc/00134/24535/22567.pdf
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Minimizing maternal effect in salmonid families mixed since eyed stages and a posteriori DNA-pedigreed ArchiMer
Haffray, Pierrick; Vandeputte, Marc; Petit, V.; Pincent, Cedric; Chatain, Beatrice; Chapuis, H.; Meriaux, J. C.; Coudurier, B.; Quillet, E.; Dupont-nivet, M..
Application of DNA parentage assignment for commercial selection in aquaculture is still rare. An experiment was performed to quantify and to minimize maternal effects on growth in rainbow trout. Six hundred families were reared until 198 day post fertilization (dpf) according to two different procedures. In the first procedure (NORM), all families were pooled at eyed stage. In the second procedure (MIN), where maternal effects due to differences in egg size were expected to be minimal, the spawns were divided into sub-groups with similar mean egg weight at eyed stage. These sub-groups were then pooled when they achieved the same mean body length (147 dpf). Genetic parameters were estimated for body weight, body length and condition factor at 198 dpf, with...
Tipo: Text Palavras-chave: Rainbow trout; Fingerprint; Maternal effect; Heritability; Growth; Triploid.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00115/22639/20395.pdf
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Transcriptome based SNP discovery and validation for parentage assignment in hatchery progeny of the European abalone Haliotis tuberculata ArchiMer
Harney, Ewan; Lachambre, Sebastien; Roussel, Sabine; Huchette, Sylvain; Enez, Florian; Morvezen, Romain; Haffray, Pierrick; Boudry, Pierre.
Selective breeding strategies require pedigree information over generations, but many species produced in aquaculture are too small to be physically tagged at early stages. Consequently, maintaining a sufficient number of separate families is often needed but costly and logistically difficult. Alternatively, parentage assignment can be obtained using DNA markers. We developed a panel of single nucleotide polymorphism (SNP) markers for the European abalone Haliotis tuberculata using an existing transcriptomic resource. An initial set of 2,176,887 SNPs was filtered to select 500 for high throughput genotyping. Of these, 298 SNPs were amplified in at least 90% of our H. tuberculata samples, consisting of a mixed family cohort (945 offspring) generated by...
Tipo: Text Palavras-chave: Abalone; Parentage assignment; Pedigree; Selective breeding; Transcriptome; SNP.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00429/54061/55396.pdf
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Genetic variation of resistance to Viral Nervous Necrosis and genetic correlations with production traits in wild populations of the European sea bass ( Dicentrarchus labrax ) ArchiMer
Khanh, Doan Quoc; Vandeputte, Marc; Chatain, Beatrice; Haffray, Pierrick; Vergnet, Alain; Breuil, Gilles; Allal, Francois.
Viral Nervous Necrosis (VNN) disease is considered as one of the most serious threats for European sea bass cultured in Mediterranean Sea, with no simple and effective procedures to treat this disease. In this study, 1472 offspring resulting from artificial full factorial mating of western Mediterranean dams with sires from four different wild populations of European sea bass (Northern Atlantic, NAT; Western Mediterranean, WEM; Northern-East Mediterranean, NEM; and Southern-East Mediterranean, SEM) were challenged by experimental infection to W80 betanodavirus strain in order to evaluate genetic variations for VNN resistance among populations and genetic correlations between VNN resistance and production traits. The results showed a large variation of VNN...
Tipo: Text Palavras-chave: VNN disease; VNN resistance; Heritability; Genetic correlation.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00385/49612/50124.pdf
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Cryopreservation of Pacific oyster ( Crassostrea gigas ) larvae: Revisiting the practical limitations and scaling up the procedure for application to hatchery ArchiMer
Labbe, Catherine; Haffray, Pierrick; Mingant, Christian; Quittet, Benjamin; Diss, Blandine; Tervit, H. Robin; Adams, Serean L.; Rimond, Flore; Suquet, Marc.
Pacific oyster Crassostrea gigas is one major species for aquaculture, and the development of breeding programs and the need for preservation of wild stock genetic resources prompted the need for larvae cryopreservation. The objective of the present study was to choose the most reliable protocol from several existing publications, to test its biological and practical limitations, and to adapt it to hatchery conditions. The selected protocol was characterized by a very slow freezing rate without seeding, and by the use of ethylene glycol and sucrose as cryoprotectant. The best survivals after thawing and rearing up to 48 h post fertilization (hpf) were obtained with larvae that were frozen at late trochophore (20 hpf) and early-D (24 hpf) stages. Increasing...
Tipo: Text Palavras-chave: Oyster; Cryobanking; D stage; Ethylene glycol; High throughput; Hatchery.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00423/53460/54354.pdf
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